Dennettia tripetala fruits (DTF) is a multi-vitamins rich edible fruit mainly consume as food and seasoning among Africans. The fruit is traditionally used to treat many diseases. The phytochemistry, preventive and healing potential of DTF on peptic ulcer were investigated in this study. The anti-peptic ulcer was assessed using the ethanol-induced ulcer model. The various biochemical markers were used for oxidative stress, lipid metabolism and histopathology of stomach lining. The sample investigated has anti-ulcer effects which significantly indicating good links between the biochemical processes. The ulcer index at 100 mg/kg and 200 mg/kg decreased, thus reducing gastrointestinal discomfort. The extract at 100 mg/kg and 200 mg/kg showed high percentage of protection against ulcers. In addition, a significant increase (p 0.05) was observed in HDL at 100 mg/kg and SOD at 200 mg/kg compared to the ethanol group. The treatment with 100 mg/kg DTF resulted in a significant (p 0.05) reduction of the ulcers scores, with an even greater (p 0.05) reduction observed at 200 mg/kg DTF.