Acta Scientifica Malaysia (ASM)

THE GENOMIC-DNA RELATIONSHIP OF SOME WILD ACCESSIONS OF COWPEA (VIGNASPP) USING UN-WEIGHTED PAIR GROUP METHOD OF ARITHMETIC MEANS (UPGMA)

October 1, 2025 Posted by sarah In asm

ABSTRACT

THE GENOMIC-DNA RELATIONSHIP OF SOME WILD ACCESSIONS OF COWPEA (VIGNASPP) USING UN-WEIGHTED PAIR GROUP METHOD OF ARITHMETIC MEANS (UPGMA)

Journal: Acta Scientifica Malaysia (ASM)

Author: V.J. Adavbiele, A.U. Omoregie and E. Akhigbe

This is an open access article distributed under the Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

DOI: 10.26480/asm.02.2025.96.99

The Genomic-DNA of five wild accessions (TVnu-59, TVnu-561, TVnu-563, TVnu-1148 and TVnu-1395) of Cowpea (Vigna species) using Un-weighted Pair Group Method of Arithmetic Means (UPGMA) was carried out at African Bioscience Laboratory, Ibadan, Oyo State, Nigeria. The samples of the five wild accessions (TVnu-561, TVnu-563, TVnu1148, TVnu-1395 and TVnu-59)were collected using molecular kits and analyzed using Single Nucleotide Polymorphism (SNP) marker.SNP generated a moderate level of polymorphism and revealed existence of similar genetic diversity among four (TVnu-561, TVnu-563, TVnu1148 and TVnu-1395) out of the five wild accessions. The three level sequences of the four wild accessions showed that two (TVnu-561 andTVnu-1395) were 40% monomorphic and three (TVnu-563, TVnu-1148 and TVnu-1395) were 60% polymorphic. However, TVnu-1395 demonstrated both monomorphism and polymorphism, a distinct character that showed its slightly similar genetic diversity with others. The Dendogram revealed the three wild accessions (TVnu-561, TVnu-563 and TVnu11480) that clustered togethershowed high degree of similar genetic diversity, formed a sub-cluster with in the one accession(TVnu-1395) with slightly similar genetic diversity. Clustering ranged from 1%-20%.TVnu-1395 which had the highest yielding characters with identified slightly genetic variations should be used to improve the others as these will enhance desirable traits such as yield.
Pages 96-99
Year 2025
Issue 2
Volume 9

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